Incubator Maintenance Primary cell culture
A guide explaining a topic
By favier margo
Disinfection / Steri-run
1
Clean the incubator
2
Remove sample and store them in a safe place
3
Take a collecting pipe
4
Clean the water pipe with 2% incidin (10ml stock solution+500ml water)
5
Place open end of the pipe in the lock drain valve
6
Open the valve and let ALL the water of the reservoir drain
7
Wipe the residual water (cloth)
8
Clean the water pipe with 2% incidin again then with water, let it dry in the air and put it to its package back.
9
Wipe the pannels (grids) with incidin solution
10
Follow the Incubator indication
11
Remove the lower panel (you can keep it in the incubator)
12
Take the HEPA-Filter out
13
Remove the filter
14
Put the the box without the filter back
15
Alarm sound - close doors
16
Run Steri-Run
17
The Run will start for 12h
If you want to stop the Steri-Run: Press STOP
18
Once Finished place the HEPA-filter again
19
Add Water to the incubator (4L+20mL of Aquacare)